A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondria

  • Truscott K
  • Voos W
  • Frazier A
 et al. 
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Transport of preproteins into the mitochondrial matrix is mediated by the presequence translocase-associated motor (PAM). Three essential subunits of the motor are known: mitochondrial Hsp70 (mtHsp70); the peripheral membrane protein Tim44; and the nucleotide exchange factor Mge1. We have identified the fourth essential subunit of the PAM, an essential inner membrane protein of 18 kD with a J-domain that stimulates the ATPase activity of mtHsp70. The novel J-protein (encoded by PAM18/YLR008c/TIM14) is required for the interaction of mtHsp70 with Tim44 and protein translocation into the matrix. We conclude that the reaction cycle of the PAM of mitochondria involves an essential J-protein.

Author-supplied keywords

  • Hsp70
  • J-protein
  • Mitochondria
  • Protein translocation
  • Saccharomyces cerevisiae

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  • Kaye N. Truscott

  • Wolfgang Voos

  • Ann E. Frazier

  • Maria Lind

  • Yanfeng Li

  • Andreas Geissler

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