LC-MS/MS determination of apixaban (BMS-562247) and its major metabolite in human plasma: An application of polarity switching and monolithic HPLC column

  • Pursley J
  • Shen J
  • Schuster A
 et al. 
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BACKGROUND: apixaban (BMS-562247) (Eliquis((R))) is a novel, orally active, selective, direct, reversible inhibitor of the coagulation factor Xa (FXa). A sensitive and reliable method was developed and validated for the measurement of apixaban (BMS-562247) and its major circulating metabolite (BMS-730823) in human citrated plasma for use in clinical testing. METHODOLOGY/RESULTS: A 0.100 ml portion of citrated plasma sample was extracted and analyzed by LC-MS/MS. Run times were approximately 3 min. The lower limit of quantification (LLOQ) was 1.00 ng/ml for BMS-562247 and 5.00 ng/ml for BMS-730823. Intra- and inter-assay precision values for replicate QC control samples were within

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  • Janice Pursley

  • Jim X. Shen

  • Alan Schuster

  • Oanh T. Dang

  • Jim Lehman

  • Michael H. Buonarati

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