Long-term in vivo imaging of normal and pathological mouse spinal cord with subcellular resolution using implanted glass windows

  • Fenrich K
  • Weber P
  • Hocine M
 et al. 
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Abstract

Key points Chronic in vivo imaging of cellular interactions within the adult spinal cord with subcellular resolution is important for understanding cellular physiology and disease progression. Previous approaches for chronic in vivo spinal cord microscopy have required surgery for each imaging session. Here we describe a novel method for implanting glass windows over the exposed spinal cords of adult mice for repeated in vivo microscopy. We show that the windows remain clear for many months after implantation, do not damage axons or blood vessels, and are useful for studying cellular dynamics after spinal cord injury. Our method represents an original technical breakthrough for scientists involved in spinal cord research and in vivo imaging, and is a useful tool for studying cellular physiology and disease progression.

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