Cytolytic activity of Met-enkephalin, an endogenous opioid peptide, was studied within the 10-7-10-17 M concentration range in K562 human erythroid leukemia cells. Cytolytic activity was determined by the trypan blue inclusion method after 13, 15 and 18 h of Met-enkephalin co-incubation with target cells. Discrete maxima of cytolytic activity were detected at concentrations of 10-9-10-10, 10-13 and 10-15 M. Cytolysis was accompanied by internucleosomal DNA fragmentation which is indicative of the mechanism of cell death being apoptosis.
CITATION STYLE
Mernenko, O. A., Blishchenko, E. Y., Mirkina, I. I., & Karelin, A. A. (1996). Met-enkephalin induces cytolytic processes of apoptotic type in K562 human erythroid leukemia cells. FEBS Letters, 383(3), 230–232. https://doi.org/10.1016/0014-5793(96)00208-6
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