Methods for monitoring autophagy from yeast to human

  • Klionsky D
  • Cuervo A
  • Seglen P
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Abstract

The increasing interest in autophagy in a wide range of organisms, accompanied by an ever-growing influx of researchers into this field, necessitates a good understanding of the methodologies available to monitor this process. In this review we discuss current approaches that can be used to follow the overall process of autophagy, as well as individual steps, from yeast to human. The majority of the review considers methods that apply to macroautophagy; however, we also consider alternative types of degradation including chaperone-mediated autophagy and microautophagy. This information is meant to provide a resource for newcomers as well as a stimulus for experienced researchers who may be prompted to develop additional assays to examine autophagy-related pathways.

Author-supplied keywords

  • Assay
  • Lysosome
  • Protein degradation
  • Protein targeting
  • Trafficking
  • Vacuole

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Authors

  • Daniel J. Klionsky

  • Ana Maria Cuervo

  • Per O. Seglen

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