Methylated 23S rRNA nucleotide m2G1835 of Escherichia coli ribosome facilitates subunit association

  • Osterman I
  • Sergiev P
  • Tsvetkov P
 et al. 
  • 18


    Mendeley users who have this article in their library.
  • 10


    Citations of this article.


Among 4.5 thousand nucleotides of Escherichia coli ribosome 36 are modified. These nucleotides are clustered in the functional centers of ribosome, particularly on the interface of large and small subunits. Nucleotide m2G1835 located on the 50S side of intersubunit bridge cluster B2 is modified by N2-methyltransferase RlmG. By means of isothermal titration calorimetry and Rayleigh light scattering, we have found that methylation of m2G1835 specifically enhances association of ribosomal subunits. No defects in fidelity of translation or interaction with translation GTPases could be ascribed to the ribosomes unmethylated at G1835 of the 23S rRNA. Methylation of G1835 was found to provide a significant advantage for bacteria at osmotic and oxidative stress. © 2011 Elsevier Masson SAS. All rights reserved.

Author-supplied keywords

  • Methylation
  • Modification
  • N2-methylated guanosine
  • Ribosome
  • rlmG

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Philipp TsvetkovAix-Marseille Université

  • Ilya A. Osterman

  • Petr V. Sergiev

  • Alexander A. Makarov

  • Alexey A. Bogdanov

  • Olga A. Dontsova

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free