Mg-ATP binding: Its modification by spermine, the relevance to cytosolic Mg2+ buffering, changes in the intracellular ionized Mg2+ concentration and the estimation of Mg2+ by 31P-NMR

  • Lüthi D
  • Günzel D
  • McGuigan J
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Abstract

It is now generally accepted that the intracellular ionized magnesium concentration ([Mg2+]i) in muscle cells is around 1 mmol 1-l; in heart muscle this means that from the total some 90-95 % is bound (see McGuigan et al. 1991a). Although binding will include sequestration by intracellular organelles, a large part of the binding is by ATP in the cytosol and an equilibrium exists in the cytosol between free ATP, ionized magnesium and Mg-ATP. The extend of this equilibrium depends on the equilibrium constant of the reaction, which is a function of pH, temperature and ionic strength. This equilibrium constant is also important in the estimation of [Mg2l]i using 31P-NMR. In this method the difference between the x and ,/ peaks of ATP is measured and from this shift and the equilibrium constant between Mg2' and ATP in the cytosol, the [Mg2+]i can be calculated (Vink, 1993).

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