Modulation of Ca2+ signalling in human vascular endothelial cells by hydrogen sulfide

  • Bauer C
  • Boyle J
  • Porter K
 et al. 
  • 23

    Readers

    Mendeley users who have this article in their library.
  • 30

    Citations

    Citations of this article.

Abstract

Hydrogen sulfide (H2S) is now recognised as an important endogenous antihypertensive molecule and is synthesised in the vasculature primarily by endothelial cystathionine γ lyase. Activity of this enzyme, and the production of other vasoactive substances by the endothelium, are subject to modulation by changes of [Ca2+]i. Here, we have used microfluorimetry to investigate whether H2S can regulate human endothelial [Ca2+]i. H2S (applied via the donor NaHS, 5-500μM) caused concentration-dependent rises of [Ca2+]iwhich were attributable to release from an ATP- and 4-CEP sensitive intracellular pool. Rises of [Ca2+]ievoked by H2S were essentially abolished by prior pool depletion. In the absence of external Ca2+, H2S slowed the decay phase of responses to cyclopiazonic acid, but this could not be attributed to the inhibition of Ca2+extrusion since the effects of H2S were at least additive with the Na+/Ca2+exchange inhibitors bepridil and SEA 0400 and the Ca2+ATPase inhibitor, carboxyeosin. In some but not all the cells, re-exposure to extracellular Ca2+following the addition and removal of H2S activated capacitative Ca2+entry (CCE), and H2S increased ATP-evoked (but not thapsigargin-evoked) CCE. Effects of H2S were not mediated by energy depletion or production of cyclic ADP ribose. Our data indicate that H2S can modulate endothelial [Ca2+]ivia multiple mechanisms, and such effects are likely to contribute to this gasotransmitter's beneficial actions. © 2009 Elsevier Ireland Ltd.

Author-supplied keywords

  • Calcium stores
  • Endothelium
  • Gasotransmitter
  • Human
  • Hydrogen sulfide
  • Intracellular calcium

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Claudia C. Bauer

  • John P. Boyle

  • Karen E. Porter

  • Chris Peers

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free