Molecular cloning and characterization of a murine pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 receptor, a murine homolog of the human immunodeficiency virus 1 entry coreceptor fusin.

  • Nagasawa T
  • Nakajima T
  • Tachibana K
 et al. 
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Pre-B-cell growth-stimulating factor/ stromal cell-derived factor 1 (PBSF/SDF-1) is a member of the CXC group of chemokines that is initially identified as a bone marrow stromal cell-derived factor and as a pre-B-cell stimulatory factor. Although most chemokines are thought to be inducible inflammatory mediators, PBSF/SDF-1 is essential for perinatal viability,. B lymphopoiesis, bone marrow myelopoiesis, and cardiac ventricular septal formation, and it has chemotactic activities on resting lymphocytes and monocytes. In this paper, we have isolated a cDNA that encodes a seven transmembrane-spanning-domain receptor, designated pre-B-cell-derived chemokine receptor (PB-CKR) from a murine pre-B-cell clone, DW34. The deduced amino acid sequence has 90% identity with that of a HUMSTSR/fusin, a human immunodeficiency virus 1 (HIV-1) entry coreceptor. However, the second extracellular region has lower identity (67%) compared with HUMSTSR/fusin. PB-CKR is expressed during embryo genesis and in many organs and T cells of adult mice. Murine PBSF/SDF-1 induced an increase in intracellular free Ca2+ in DW34 cells and PB-CKR-transfected Chinese hamster ovary (CHO) cells, suggesting that PB-CKR is a functional receptor for murine PBSF/SDF-1. Murine PBSF/ SDF-1 also induced Ca2+ influx in fusin-transfected CHO cells. On the other hand, considering previous results that HIV-1 does not enter murine T cells that expressed human CD4, PB-CKR may not support HIV-1 infection. Thus, PB-CKR will be an important tool for functional mapping of HIV-1 entry coreceptor fusin and for understanding the function of PBSF/SDF-1 further.

Author-supplied keywords

  • Adult
  • Amino Acid Sequence
  • Animals
  • Chemokine CXCL12
  • Chemokines, CXC
  • Cloning, Molecular
  • Cricetinae
  • Cytokines
  • Cytokines: genetics
  • Humans
  • Membrane Proteins
  • Membrane Proteins: genetics
  • Membrane Proteins: metabolism
  • Mice
  • Molecular Sequence Data
  • Receptors, CXCR4
  • Receptors, Cytokine
  • Receptors, Cytokine: genetics
  • Receptors, Cytokine: metabolism
  • Receptors, HIV
  • Receptors, HIV: genetics
  • Receptors, HIV: metabolism
  • Sequence Analysis
  • Signal Transduction

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  • T Nagasawa

  • T Nakajima

  • K Tachibana

  • H Iizasa

  • C C Bleul

  • O Yoshie

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