A new rapid method for clostridium difficile DNA extraction and detection in stool: Toward point-of-care diagnostic testing

  • Freifeld A
  • Simonsen K
  • Booth C
 et al. 
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Abstract

We describe a new method for the rapid diagnosis of Clostridium difficile infection, with stool sample preparation and DNA extraction by heat and physical disruption in a single-use lysis microreactor (LMR), followed by a rapid PCR amplification step. All steps can be accomplished in

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