Fluorescence microscopy is one of the most powerful tools for elucidating the cellular functions of proteins and other molecules. In many cases, the function of a molecule can be inferred from its association with specific intracellular compartments or molecular complexes, which is typically determined by comparing the distribution of a fluorescently labeled version of the molecule with that of a second, complementarily labeled probe. Although arguably the most common application of fluorescence microscopy in biomedical research, studies evaluating the "colocalization" of two probes are seldom quantified, despite a diversity of image analysis tools that have been specifically developed for that purpose. Here we provide a guide to analyzing colocalization in cell biology studies, emphasizing practical application of quantitative tools that are now widely available in commercial and free image analysis software. © 2011 the American Physiological Society.
CITATION STYLE
Dunn, K. W., Kamocka, M. M., & McDonald, J. H. (2011, April). A practical guide to evaluating colocalization in biological microscopy. American Journal of Physiology - Cell Physiology. https://doi.org/10.1152/ajpcell.00462.2010
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