Preparation and characterization of laminin-derived peptide AG73-coated liposomes as a selective gene delivery tool.

  • Negishi Y
  • Omata D
  • Iijima H
 et al. 
  • 17

    Readers

    Mendeley users who have this article in their library.
  • 13

    Citations

    Citations of this article.

Abstract

Targeted gene delivery to cancer cells is considered as a promising strategy for cancer therapy. Since, several targeting ligands have been studied for cancer gene therapy, such as transferrin, folate, anisamide, RGD-peptide, and antibodies. We have focused on AG73 peptide, which is derived from the globular domain of the laminin α1 chain. AG73 peptide is known as a ligand for syndecans, one of the major heparin sulfate-containing transmembrane proteoglycans. Syndecan-2 is highly expressed in various cancer cells and plays a role in angiogenesis. In this study, we prepared AG73-labeled polyethyleneglycol-modified liposomes (AG73-PEG liposomes) for gene delivery tool to syndecan-2 overexpressing cancer cells, and assessed the characterization of AG73-PEG liposomes. We confirmed the conjugation of AG73 peptide to PEG liposomes by reverse-phase high-performance liquid chromatography analysis. Electron microscopy analysis showed that monodiseperse AG73-labeled lipsomes were prepared. We also assessed the gene transfection efficiency of AG73-PEG liposomes in syndecan-2 overexpressing cancer cells or syndecan-2 less expressing cancer cells. As a result, AG73-mediated liposomal gene transfection efficiency was increased by 100-fold in syndecan-2 overexpressing cancer cells compared to syndecan-2 less expressing cancer cells. These results suggested that AG73-PEG liposomes were successfully prepared from a point of view of the modification of AG73 peptide to PEG-liposomes and the particle size of liposomes, which presented nano size. Furthermore, our results suggest that AG73-PEG liposomes can be a useful targeted gene delivery vehicle for syndecan-2 overexpressing cancer cells.

Author-supplied keywords

  • Cell Line
  • Chromatography
  • DNA
  • DNA: administration & dosage
  • DNA: therapeutic use
  • Electron
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Therapy: methods
  • High Pressure Liquid
  • Humans
  • Laminin
  • Liposomes
  • Liposomes: administration & dosage
  • Liposomes: chemical synthesis
  • Microscopy
  • Neoplasms
  • Neoplasms: drug therapy
  • Neoplasms: metabolism
  • Particle Size
  • Peptide Fragments
  • Syndecan-2
  • Syndecan-2: metabolism
  • Transfection
  • Transfection: methods
  • Tumor
  • ag73 peptide
  • can selectively deliver therapeu-
  • delivery vehicles or vectors
  • gene delivery
  • in gene therapy
  • it is necessary that
  • liposome
  • syndecan
  • the development of

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Yoichi Negishi

  • Daiki Omata

  • Hiroshi Iijima

  • Nobuhito Hamano

  • Yoko Endo-Takahashi

  • Motoyoshi Nomizu

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free