Protection of reoxygenated cardiomyocytes against sarcolemmal fragility: The role of glutathione

  • Obermayr R
  • Schlüter K
  • Schäfer M
 et al. 
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Abstract

This study addressed the question of whether the sarcolemmal fragility of cardiomyocytes after anoxia and subsequent reoxygenation can be altered by modulation of the cellular glutathione state. Isolated ventricular cardiomyocytes (from adult rats) were exposed to 120 min anoxia and subsequently to 30 min reoxygenation. Osmotic stress was generated by reduction of medium osmolarity from 270 to 80 mosmol/l and sarcolemmal fragility assessed by the leakage of lactate dehydrogenase (LDH). Under normoxic conditions 6.7+/-1.0 % of total LDH activity was found extracellularly. Hyposmolar reoxygenation, but not hypoosmolar anoxia, increased LDH release (17.9+/-2.7% of total, P

Author-supplied keywords

  • Free radicals
  • Ischaemia
  • Myocytes
  • Reperfusion

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