Protein and peptide fractionation, enrichment and depletion: Tools for the complex proteome

  • Ly L
  • Wasinger V
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Abstract

The identification, quantitation and global characterisation of all proteins within a given proteome are extremely challenging. This is due to the absolute detection limits of technology as well as the dynamic range in expression of proteins; and the extreme diversity and heterogeneity of the proteome. To overcome such issues, the use of separation technologies has played a critical role in reducing sample complexity. To date, a plethora of chromatographic and electrophoretic fractionation tools have evolved over the years assisting in simplifying complex protein and peptide mixtures. Here, we review a range of these technologies highlighting the challenges of protein and peptide analysis in the context of proteome research and some of the advantages and disadvantages of present techniques.

Author-supplied keywords

  • Chromatography
  • Electrophoresis
  • Fractionation
  • Protein
  • Proteome
  • Technology

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Authors

  • Linda Ly

  • Valerie C. Wasinger

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