Doxorubicin-DNA association has been studied by quantitative microspectrofluorometry. Fluorescence emission spectra from a microvolume of single living cell nuclei treated with doxorubicin have been analyzed in terms of difference in spectral shape and fluorescence yield between free and DNA-bound drug. Contribution of each spectral component to the total signal was calculated by least-squares linear regression. With this method of analysis, total drug concentration has been determined with an error of less than 10%. Moreover, the uptake into the nucleus has been studied in a non destructive way, avoiding use of 14C-labelled drug. Kinetic studies of drug accumulation into the nuclei were conducted on sensitive and resistant cells. © 1988.
CITATION STYLE
Gigli, M., Doglia, S. M., Millot, J. M., Valentini, L., & Manfait, M. (1988). Quantitative study of doxorubicin in living cell nuclei by microspectrofluorometry. BBA - Gene Structure and Expression, 950(1), 13–20. https://doi.org/10.1016/0167-4781(88)90068-1
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