Recombinant protein production by In Vivo polymer inclusion display

  • Grage K
  • Peters V
  • Rehm B
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Abstract

A novel approach to produce purified recombinant proteins was established. The target protein is produced as polyhydroxyalkanoate (PHA) synthase fusion protein, which mediates intracellular formation of PHA inclusions displaying the target protein. After isolation of the PHA inclusions, the pure target protein was released by simple enterokinase digestion.

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Authors

  • Katrin Grage

  • Verena Peters

  • Bernd H A Rehm

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