We describe a highly polymorphic microsatellite panel for identifying individual leopards using DNA from scat. After successfully screening 16 published microsatellites, we optimized a panel of 7 microsatellites that yields a Probability of Identity between siblings value of 5.24E-04. We used this panel to identify 217 individuals from 287 leopard scats collected from five tiger-reserves in Central India. We identified 101 males and 92 females by amplifying a fragment of the Amelogenin protein gene. This panel will be helpful to study genetic structure, gene flow, relatedness and sex ratio of leopards. © 2012 Springer Science+Business Media B.V. (outside the USA).
CITATION STYLE
Dutta, T., Sharma, S., Maldonado, J. E., Wood, T. C., & Seidensticker, J. (2012). A reliable method for individual identification and gender determination of wild leopards (Panthera pardus fusca) using non-invasive samples. Conservation Genetics Resources, 4(3), 665–667. https://doi.org/10.1007/s12686-012-9618-5
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