RNA Therapeutics

  • Algae T
  • Atlantic M
  • States U
 et al. 
  • 1

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Abstract

RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the mechanisms by which these modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of techniques, including oligonucleotide-affinity selection, site-specific cleavage and radiolabeling, nuclease digestion, and thin layer chromatography.

Author-supplied keywords

  • 04
  • 1
  • 10
  • 2014
  • 2305-5839
  • 3-nitrotyrosine
  • 30
  • 3978
  • ABC transporters
  • ABCB1
  • ATP binding cassette transporter
  • Adhesion
  • Alternative Splicing
  • Animals
  • Antineoplastic Agents
  • Antineoplastic Agents: pharmacology
  • Antineoplastic Agents: therapeutic use
  • Antitumor antibodies
  • Artifacts
  • BAC
  • Base Sequence
  • Bioinformatics
  • CELLBIO
  • Cadherins
  • Cadherins: biosynthesis
  • Cadherins: genetics
  • Cadherins: physiology
  • Cancer
  • Cancer Stem Cell
  • Cancer Therapy
  • Cancer progenitor cells
  • Cancer stem cell
  • Cancer-initiating cell
  • Carcinogenesis
  • Carcinogenesis: genetics
  • Carcinogenesis: metabolism
  • Cell Fractionation
  • Cell Transformation
  • Cell adhesion
  • Cell migration
  • Cells
  • Chemotherapy
  • Collateral sensitivity
  • Combination therapy
  • Cultured
  • DNA Mutational Analysis
  • DNA Replication Timing
  • Drug Resistance
  • Drug metabolism
  • Drug resistance
  • Drug transporters
  • E. coli
  • Epigenesis
  • Epigenetics
  • Epithelial Cells
  • Epithelial Cells: cytology
  • Epithelial Cells: pathology
  • Epithelial Cells: physiology
  • Epithelial mesenchymal transition
  • Epithelial to Mesenchymal Transition
  • Epithelial-Mesenchymal Transition
  • Epithelial-Mesenchymal Transition: drug effects
  • Epithelial-mesenchymal transition
  • Escherichia coli
  • Exome
  • Exome: genetics
  • False Positive Reactions
  • Functional genomics
  • GSK461364
  • Gene Expression
  • Gene Expression Regulation
  • Genetic
  • Genetic Heterogeneity
  • Genome
  • HUMDISEASE
  • Helix-Loop-Helix Motifs
  • Host-Pathogen Interactions
  • Human
  • Human: genetics
  • Humans
  • Immune cells
  • Immune suppression
  • Immune surveillance
  • Inflammation
  • Intermediate filament
  • Intermediate filaments
  • Invasion
  • Kinase
  • Lung Neoplasms
  • Lung Neoplasms: genetics
  • Lung cancer
  • Mass Spectrometry
  • Membrane protein
  • Mesoderm
  • Mesoderm: chemistry
  • Mesoderm: pathology
  • Mesoderm: physiology
  • Metabolome
  • Metabolomics
  • Metastasis
  • Methylation
  • Mice
  • Microenvironment
  • Modulators
  • Molecular Sequence Data
  • Multidrug resistance
  • Mutation
  • Mutation Rate
  • Mutation: genetics
  • Nanomedicine
  • Nanomedicine: methods
  • Neoplasm
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Neoplasms
  • Neoplasms: classification
  • Neoplasms: drug therapy
  • Neoplasms: genetics
  • Neoplasms: metabolism
  • Neoplasms: pathology
  • Neoplastic
  • Neoplastic Stem Cells
  • Neoplastic Stem Cells: cytology
  • Neoplastic Stem Cells: drug effects
  • Neoplastic Stem Cells: metabolism
  • Neoplastic Stem Cells: pathology
  • Nucleobond
  • Oncoantigens
  • Oncogene addiction
  • Oncogenes
  • Oncogenes: genetics
  • Origin of cancer
  • Phosphatase
  • Phosphatidylinositol 3-Kinases
  • Phosphatidylinositol 3-Kinases: metabolism
  • Phosphorylation
  • Polo-like kinase 1
  • Prevention
  • Prognosis
  • Promoter Regions
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins c-akt: metabolism
  • R Medicine (General)
  • RNA
  • RNA interference
  • Reproducibility of Results
  • Review
  • STEMCELL
  • Sample Size
  • Self-renewal
  • Sequence Deletion
  • Signal Transduction
  • Signaling
  • Squamous Cell
  • Squamous Cell: genetics
  • Stem Cell State
  • Stem cell
  • Synthetic lethality
  • Therapy
  • Tissue Distribution
  • Transcription Factors
  • Transcription Factors: metabolism
  • Tumor metastasis
  • Tumor microenvironment
  • Tumor vaccine
  • Tumorigenesis
  • Tyrosine kinase inhibitors
  • Untranslated
  • Untranslated: genetics
  • Untranslated: metabolism
  • Vimentin
  • X-ray crystallography
  • a_Method
  • abc
  • abc superfamily
  • abcc1
  • abcg2
  • accepted for publication sep
  • acute lung injury
  • ampicillin
  • ampk
  • apoptosis
  • asc
  • atp
  • bacterial artificial chromosome
  • bcrp
  • bio
  • blood
  • bmi1
  • cancer
  • cancer chemotherapy
  • cancer microenvironment
  • cancer stem cells
  • chemotherapy
  • chloramphenicol
  • circulating tumor cells
  • cloning site
  • copy number
  • cosmid
  • criteria for choosing a cloning vector
  • ctcs
  • delivery
  • design
  • diversity
  • dna purification
  • doi
  • drug efflux
  • drug efflux transporters
  • drug resistance
  • drug therapy
  • drug transport
  • dx
  • elisa
  • emt
  • energy metabolism
  • epithelial
  • epithelial mesenchymal transition
  • epithelial-mesenchymal transition
  • evolution
  • expanding the tropism of
  • gDNA
  • gene regulation
  • gene therapy
  • genetic polymorphism
  • genomic dna
  • glycoproteins
  • http
  • human atp-binding cassette
  • human genome
  • hydrolysis
  • hyopxia
  • il-1β
  • incompatibility
  • insert size
  • invasion
  • isolation
  • issn
  • j
  • kanamycin
  • lambda vectors
  • lentiviral vector
  • lentivirus
  • lentiviruses through
  • lung neoplasms
  • mdr
  • medical research
  • mesenchymal transition
  • metastasis
  • microrna
  • midiprep
  • mrp1
  • multidrug resistance
  • multidrug resistance protein
  • neoplasm metastasis
  • nitric oxide
  • org
  • p-glycoprotein
  • peroxynitrite
  • pgc-1 α
  • plasmid
  • porphyromonas gingivalis
  • prognosis
  • protein
  • reactive oxygen species
  • rho kinase
  • rna interference
  • rnai
  • rock
  • selectable marker
  • selection
  • sepsis
  • short hairpin rna
  • shrna
  • sirna
  • sirt1
  • small interfering rna
  • specialized plasmid function
  • stemness
  • submitted aug 13
  • tetracycline
  • therapy
  • thp1
  • transfection
  • transporter gene family
  • twist1
  • vector tropism
  • view this article at
  • western blot

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Authors

  • The Marine Algae

  • Mid Atlantic

  • United States

  • South Atlantic States

  • Marine Biology

  • Multiple Sequence Alignment

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