RNA-Seq analysis and whole genome DNA-binding profile of the Vibrio cholerae histone-like nucleoid structuring protein (H-NS)

  • Ayala J
  • Wang H
  • Benitez J
 et al. 
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The data described in this article pertain to the genome-wide transcription profiling of a Vibrio cholerae mutant lacking the histone-like nucleoid structuring protein (H-NS) and the mapping of the H-NS chromosome binding sites (Wang et al., 2015 [1]; Ayala et al., 2015 [2]). H-NS is a nucleoid-associated protein with two interrelated functions: organization of the bacterial nucleoid and transcriptional silencing (Dorman, 2013 [3]). Both functions require DNA binding and protein oligomerization (Spurio et al., 1997. [4]; Dame et al., 2001 [5]). H-NS commonly silences the expression of virulence factors acquired by lateral gene transfer (Navarre et al., 2006 [6]). The highly pleiotropic nature of hns mutants in V. cholerae indicates that H-NS impacts a broad range of cellular processes such as virulence, stress response, surface attachment, biofilm development, motility and chemotaxis. We used a V. cholerae strain harboring a deletion of hns and a strain expressing H-NS tagged at the C-terminus with the FLAG epitope to generate datasets representing the hns transcriptome and DNA binding profile under laboratory conditions (LB medium, 37. °C). The datasets are publicly available at the Gene Expression Omnibus (GEO) repository (. http://www.ncbi.nlm.nih.gov/geo/) with accession numbers GSE62785 and GSE64249.

Author-supplied keywords

  • Cholera
  • H-NS
  • Histone-like nucleoid structuring protein
  • Nucleoid associated protein
  • Vibrio cholerae

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