Journal article

Semaphorin3D Guides Retinal Axons along the Dorsoventral Axis of the Tectum

Liu Y, Berndt J, Su F, Tawarayama H, Shoji W, Kuwada J, Halloran M ...see all

The Journal of Neuroscience, vol. 24, issue 2 (2004) pp. 310-318

  • 36


    Mendeley users who have this article in their library.
  • 49


    Citations of this article.
Sign in to save reference


We examined the role of Sema3D, a semaphorin of previously unknown function, in guiding retinal ganglion cell (RGC) axons to the optic tectum in the developing zebrafish. Sema3D is expressed more strongly in the ventral versus dorsal tectum, suggesting that it may participate in guiding RGC axons along the dorsoventral axis of the tectum. Ubiquitous misexpression of Sema3D in transgenic zebrafish inhibits ventral but not dorsal RGC axon growth. In addition, ventral RGC axons avoid or stop at individual cells misexpressing Sema3D along their pathway. Sema3D ubiquitous misexpression at later stages also causes ventral RGC axon arbors to spread more widely along the dorsoventral axis of the tectum. Knock-down of Sema3D with morpholino antisense causes ventral RGC axons to extend aberrantly into the ventral tectum. These results suggest that Sema3D in the ventral tectum normally acts to inhibit ventral RGCs from extending into ventral tectum, ensuring their correct innervation of dorsal tectum.

Author-supplied keywords

  • neuropilin
  • retinal axon guidance
  • retinotectal
  • semaphorin
  • topographic map
  • transgenic zebrafish

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Yan Liu

  • Jason Berndt

  • Fengyun Su

  • Hiroshi Tawarayama

  • Wataru Shoji

  • John Y. Kuwada

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free