Journal article

A set of compatible tac promoter expression vectors

Dykxhoorn D, St. Pierre R, Linn T ...see all

Gene, vol. 177, issue 1-2 (1996) pp. 133-136

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Abstract

A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacI(q) gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.

Author-supplied keywords

  • Copy number
  • Gene regulation
  • Incompatibility group
  • LacI gene
  • Recombinant DNA
  • pUC19 polylinker
  • β-galactosidase

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Authors

  • Derek M. Dykxhoorn

  • Rebecca St. Pierre

  • Thomas Linn

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