Simultaneous quantitation of delta-9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum by GC/MS using deuterated internal standards and its application to a smoking study and forensic cases.

  • Moeller M
  • Doerr G
  • Warth S
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Abstract

A new procedure for the simultaneous detection of delta-9-tetrahydrocannabinol (THC) and its major metabolite, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in serum has been evaluated. The method combines rapid, efficient, solid-phase extraction and simple derivatization by methylation. Analysis and quantitation is performed by gas chromatography/mass spectrometry (GC/MS) using deuterated cannabinoids as internal standards (IS). Reproducibility and sensitivity of the method are good. The procedure is applied to serum specimens collected from a smoking study with 24 volunteers and 212 forensic cases. Results are interpreted based upon the current knowledge about THC metabolism and pharmacokinetics.

Author-supplied keywords

  • Calibration
  • Dronabinol
  • Dronabinol: analogs & derivatives
  • Dronabinol: blood
  • Dronabinol: pharmacokinetics
  • Female
  • Forensic Medicine
  • Forensic Medicine: instrumentation
  • Forensic Medicine: methods
  • Gas Chromatography-Mass Spectrometry
  • Half-Life
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Marijuana Smoking
  • Marijuana Smoking: blood
  • Reproducibility of Results

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Authors

  • M R Moeller

  • G Doerr

  • S Warth

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