In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers.

  • Jacob J
  • Kelsoe G
  • 64


    Mendeley users who have this article in their library.
  • 360


    Citations of this article.


In the genetically restricted response that follows immunization with (4-hydroxy-3-nitrophenyl)acetyl coupled to protein carriers, two distinct populations of B cells are observed in the spleens of C57BL/6 mice. By 48 h postimmunization, foci of antigen-binding B cells appear along the periphery of the periarteriolar lymphoid sheaths. These foci expand to contain large numbers of antibody-forming cells that neither bind the lectin, peanut agglutinin, nor mutate the rearranged immunoglobulin variable region loci. Germinal centers containing peanut agglutinin-positive B cells can be observed by 96-120 h after immunization. Although specific for the immunizing hapten, these B cells do not produce substantial amounts of antibody, but are the population that undergoes somatic hypermutation and affinity-driven selection. Both focus and germinal center populations are pauciclonal, founded, on average, by three or fewer B lymphocytes. Despite the highly specialized roles of the focus (early antibody production) and germinal center (higher affinity memory cells) B cell populations, analysis of VH to D to JH joins in neighboring foci and germinal centers demonstrate that these B cell populations have a common clonal origin.

Author-supplied keywords

  • Animals
  • B-Lymphocytes
  • B-Lymphocytes: chemistry
  • B-Lymphocytes: immunology
  • Base Sequence
  • Clone Cells
  • DNA
  • Female
  • Haptens
  • Haptens: immunology
  • Immunoenzyme Techniques
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Heavy Chains: genetics
  • Immunoglobulin Heavy Chains: immunology
  • Lymphoid Tissue
  • Lymphoid Tissue: cytology
  • Lymphoid Tissue: immunology
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nitrophenols
  • Nitrophenols: immunology
  • Phenylacetates
  • Sequence Homology, Nucleic Acid

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • J Jacob

  • G Kelsoe

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free