Understanding the behaviour of Cryptosporidium oocysts in the environment is critical for developing improved watershed management practices for protection of the public from waterborne cryptosporidiosis. Analytical methods of improved specificity and sensitivity are essential to this task. We developed a nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay that allows detection of a single oocyst in environmental samples and differentiates the human pathogen Cryptosporidium parvum from other Cryptosporidium species. We tested our method on surface water and animal faecal samples from the Wachusett Reservoir watershed in central Massachusetts, USA from March 1999 to January 2001 and February 2000 to January 2001 and. We also directly compared results from our method with those from the immunofluorescence microscopy assay recommended in the Information Collection Rule. Our results suggest that immunofluorescence microscopy may not be a reliable indicator of public health risk for waterborne cryptosporidiosis. Molecular and environmental data identify both wildlife and dairy farms as sources of oocysts in the watershed, implicate times of cold water temperatures as high-risk periods for oocyst contamination of surface waters and suggest that not all oocysts in the environment pose a threat to public health.
Mendeley saves you time finding and organizing research
Choose a citation style from the tabs below