Spontaneous hybridization between Pinus sylvestris L. and P. mugo Turra in Slovakia

  • Kormutak A
  • Demankova B
  • Gömöry D
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Molecular evidence for spontaneous hybridization between Pinus sylvestris L. and R mugo Turra in the putative hybrid swarm populations of the species in Slovakia was provided based on PCR-RFLP analysis of the cpDNA trnV-trnH region. Species-specific restriction profiles generated by Hinf I digests of the cpDNA products reliably identified R sylvestris and R mugo haplotypes of the embryos from open pollination. Simultaneous analysis of the respective cpDNA region in megaga-metophytes and embryos of individual seeds along with needles of a given maternal tree has enabled to score either the R sylvestris or R mugo haplotypes in the embryos illustrating hybridization patterns between the two species. Data obtained in this way indicate a relatively extensive hybridization which takes place between R sylvestris and R mugo. The extent of hybridization varied among populations as evidenced by the 41.1-58.7% proportion of hybrid embryos registered on the locality Habovka, and by the 8.3% and 2.7% proportions of hybrid embryos on the localities Tisovnica and Sucha Hora, respectively. The approach itself is recommended as a convenient method for monitoring the hybridization patterns in sympatric zones of the studied pine species.

Author-supplied keywords

  • Chloroplast DNA restriction analysis
  • Hybridization
  • P. mugo Turra
  • Pinus sylvestris L.

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  • SGR: 46449096376
  • PMID: ISI:000257243900005
  • SCOPUS: 2-s2.0-46449096376
  • ISBN: 0037-5349
  • PUI: 351929862
  • ISSN: 00375349


  • A. Kormutak

  • B. Demankova

  • D. Gömöry

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