The cyanobacterium Synechocystis PCC 6803 grown under short-term iron-deficient conditions assembles a supercomplex consisting of a trimeric Photosystem I (PSI) complex encircled by a ring of 18 IsiA complexes. Furthermore, it has been shown that single or double rings of IsiA with up to 35 copies in total can surround monomeric PSI. Here we present an analysis by electron microscopy and image analysis of the various PSI-IsiA supercomplexes from a Synechocystis PCC 6803 mutant lacking the PsaL subunit after short- and long-term iron-deficient growth. In the absence of PsaL, the tendency to form complexes with IsiA is still strong, but the average number of complete rings is lower than in the wild type. The majority of IsiA copies binds into partial double rings at the side of PsaF/J subunits rather than in complete single or double rings, which also cover the PsaL side of the PSI monomer. This indicates that PsaL facilitates the formation of IsiA rings around PSI monomers but is not an obligatory structural component in the formation of PSI-IsiA complexes. © 2004 Elsevier B.V. All rights reserved.
CITATION STYLE
Kouřil, R., Yeremenko, N., D’Haene, S., Oostergetel, G. T., Matthijs, H. C. P., Dekker, J. P., & Boekema, E. J. (2005). Supercomplexes of IsiA and Photosystem I in a mutant lacking subunit PsaL. Biochimica et Biophysica Acta - Bioenergetics, 1706(3), 262–266. https://doi.org/10.1016/j.bbabio.2004.11.008
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