Time-Dependent Alterations of Select Genes in Streptozotocin-Induced Diabetic Rat Bladder

  • Gray M
  • Wang C
  • Sacks M
 et al. 
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Abstract

Objectives: To investigate time-course changes in the expression of select genes and extracellular matrix proteins in the bladder of rats with streptozotocin-induced diabetes, so as to examine the mechanisms underlying changes in mechanical properties of the bladder due to diabetic cystopathy. Methods: Female Sprague-Dawley rats were injected with streptozotocin (65 mg/kg). Rats that were fed with 5% sucrose in drinking water served as diuretic controls, in addition to normal control rats. At the end of 2, 4, and 8 weeks, total ribonucleic acid (RNA) isolated from the detrusor layer of the bladders was reverse transcribed, and then complementary deoxyribonucleic acid was amplified with polymerase chain reaction primer sets for type I collagen, type III collagen, tropoelastin, and transforming growth factor beta 1 (TGF-beta-1). Collagen and elastin contents of the bladders were quantified with commercially available assays. Results: Both diabetic and diuretic rat bladders exhibited significantly (P

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