Transcription factories are nuclear subcompartments that remain in the absence of transcription

  • Mitchell J
  • Fraser P
  • 187


    Mendeley users who have this article in their library.
  • 155


    Citations of this article.


Nascent transcription occurs at nuclear foci of concentrated, hyperphosphorylated RNA polymerase II (RNAPII). We investigate RNAPII localization, distal gene co-association, and Hbb locus conformation during inhibition of transcription. Our results show distal active genes remain associated with RNAPII foci and each other in the absence of elongation. When initiation is inhibited, active genes dissociate from RNAPII foci and each other, suggesting initiation is necessary to tether distal active genes to shared foci. In the absence of transcription RNAPII foci remain, indicating they are not simple accumulations of RNAPII on transcribed genes but exist as independent nuclear subcompartments.

Author-supplied keywords

  • Chromosome conformation capture
  • Fluorescence in situ hybridization
  • Locus control region
  • Nuclear organization
  • RNA polymerase II

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free