Transformation of azuki bean by Agrobacterium tumefaciens

  • Yamada T
  • Teraishi M
  • Hattori K
 et al. 
  • 8


    Mendeley users who have this article in their library.
  • 34


    Citations of this article.


Stable transformation and regeneration was developed for a grain legume, azuki bean (Vigna angularis Willd. Ohwi & Ohashi). Two constructs containing the neomycin phosphotransferase II gene (nptII) and either the beta - glucuronidase (GUS) gene or the modified green fluorescent protein [sGFP(S65T)] gene were introduced independently via Agrobacterium tumefaciens-mediated transformation. After 2 days of co-cultivation on MS medium supplemented with 100 muM acetosyringone and 10 mg 1(-1) 6-benzyladenine, seedling epicotyl explants were placed on regeneration medium containing 100 mg 1(-1) kanamycin. Adventitious shoots developing from explant calli were excised onto rooting medium containing 100 mg 1(-1) kanamycin. Rooted shoots were excised and repeatedly selected on the same medium containing kanamycin. Surviving plants were transferred to soil and grown in a green house to produce viable seeds. This process took 5 to 7 months after co- cultivation. Molecular analysis confirmed the stable integration and expression of foreign genes.

Author-supplied keywords

  • 6-benzyladenine
  • Acetosyringone
  • Co-cultivation
  • Grain legume
  • Green fluorescent protein
  • Neomycin phosphotransferase
  • Transgenic plant
  • Vigna angularis

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free