Transmembrane protein structure: Spin labeling of bacteriorhodopsin mutants

  • Altenbach C
  • Marti T
  • Khorana H
 et al. 
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Single cysteine residues were introduced at 18 consecutive positions in bacterial rhodopsin by mutation and reacted with a specific spin label. By measuring the relative collision frequency with freely diffusing oxygen, residues in contact with the lipid bilayer could clearly be distinguished from those involved in protein association.

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  • Christian Altenbach

  • Thomas Marti

  • H. Gobind Khorana

  • Wayne L. Hubbell

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