Transport of glutathione(GSH) into mitochondria was observed when mitochondria in state 4 respiration were incubated with high concentrations of GSH. This transport was suppressed by antimycin A or dicyclohexyl-carbodiimide, or in state 3 respiration. Upon dissipation of the proton gradient by a proton ionophore, mitochondrial GSH was released into the medium. GSH moved freely across the proton-permeated mitochondrial membrane, its movement depending only on the GSH gradient across the inner membrane. These results indicate that there is a transport system for GSH in the mitochondrial membrane, and that a proton gradient is necessary to maintain GSH in the matrix, and to transport GSH into mitochondria. © 1990.
CITATION STYLE
Kurosawa, K., Hayashi, N., Sato, N., Kamada, T., & Tagawa, K. (1990). Transport of glutathione across the mitochondrial membranes. Biochemical and Biophysical Research Communications, 167(1), 367–372. https://doi.org/10.1016/0006-291X(90)91774-M
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