Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis

  • Vinothkumar K
  • Bhardwaj A
  • Ramamurthy T
 et al. 
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Abstract

A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology. © 2013 Elsevier Inc.

Author-supplied keywords

  • Diagnostics
  • Epidemiology
  • Haem-utilising gene
  • Haemolysin gene
  • Toxin-regulatory gene

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Authors

  • Kittappa Vinothkumar

  • Ashima Kushwaha Bhardwaj

  • Thandavarayan Ramamurthy

  • Swapan Kumar Niyogi

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