A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology. © 2013 Elsevier Inc.
CITATION STYLE
Vinothkumar, K., Bhardwaj, A. K., Ramamurthy, T., & Niyogi, S. K. (2013). Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis. Diagnostic Microbiology and Infectious Disease, 76(4), 526–528. https://doi.org/10.1016/j.diagmicrobio.2013.04.005
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