We analyzed the mRNA diversity of genes after inducing neuronal differentiation in human NT2 teratocarcinoma cells using all-trans retinoic acid (RA). DNA microarray analyses of cells treated with RA identified 358 RA-responsive genes. mRNA diversity analysis revealed that 274 genes produced multiple protein-coding transcripts by alternative splicing. Among these 274 genes, we chose 26 genes that showed AS in their C-terminus and 12 transcription factor genes for further analysis. By using transcript-specific primers, we performed quantitative real-time PCR analysis to examine the expression profiles of all the protein-coding transcripts. Consequently, we identified genes which showed different RA-induced changes in the expression of their protein-coding transcripts. © 2010 Federation of European Biochemical Societies.
CITATION STYLE
Wakamatsu, A., Imai, J. ichi, Watanabe, S., & Isogai, T. (2010). Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells. FEBS Letters, 584(18), 4041–4047. https://doi.org/10.1016/j.febslet.2010.08.024
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