Antitumor Activity and Immunological Property of Polysaccharides from the Mycelium of Liquid-Cultured Grifola frondosa.

Abstract

Asystematic from the mycelium method for the fractionation and purification of antitumor of liquid-cultured Grifola Frondosa was established. polysaccharide Twenty-three fractions polysaccha- ride fractions were obtained. FI0-a-α, FI0-a-β, FA-1 and FA-2-b-α in water-soluble fractions showed better antitumor activity against Sarcoma 180/mice, FIII-1-a, FIII-1-b, FIII-2-a, FIII-2-b and FIII-2-c in water-insoluble fractions markedly inhibited the growth of Sarcoma l80/mice. In addition, of each of FI0-a, FIII-1-a, FIII-1一b, FIII-1-c, FIII-2-a, FIII-2-b, FIII-2-c to mice could administration These results suggest C3 release from macrophages. that cause an evident increase in antigenic active polysaccharide fractions, which were considered to be heteroglycan or heteroglycan-protein complexes, can depress or reduce tumor growth by activating the immune system as a biological response modifier (BRM).