Blood Sample Collection and Handling

Abstract

The information obtained from collection and analysis of blood samples often plays a critical role in the diagnostic process. Improper handling issues including failure to invert collection tubes adequately to mix the anticoagulant and blood, improper or delayed centrifugation, inadequate filling of blood tubes, improper storage of collected samples, and other handling errors may also have a negative impact on sample integrity and quality. Several safety issues need to be considered during collection and handling of blood samples. Venous blood samples may be collected by direct insertion of a needle into a vein (venipuncture) or by aspiration of blood through a peripheral or central IV catheter. The discard method involves drawing a volume of blood from the catheter and discarding it to minimize the risk of sample dilution or interfering substances. Blood cultures may be helpful to identify causative agents in patients with suspected bacteremia.