Fluorescent imaging of drosophila melanogaster sperm in the reproductive tract: A new model of flagellar motility

Abstract

The sperm of Drosophila melanogaster stands out because of its enormous length and perplexing movements that break some norms of flagella. Most flagella with analyzed motility, ranging from flagella of Chlamydomonas to sperm of marine invertebrates and vertebrates, are from a few microns to at most 208 μm in length. Most flagella propagate waves in a constant direction, starting at the base of the flagellum and moving toward the tip (base-to-tip waves). In contrast, the fly sperm is 1.9-mm long and it propagates waves in base-to-tip or tip-to-base direction, generating head-leading or tail-leading movement. Of the two movement orientations, the sperm choose one or the other for a particular movement along the convoluted path leading to fertilization. For example, the sperm enter the seminal receptacle (SR) for storage with a tail-leading movement, but exit it for fertilization with a head-leading movement. Moreover, the sperm move with unusual waveforms. A modified sinusoidal or arc-line waveform generates semistationary movement - moving but staying at a general area - within temporary reservoirs (ejaculatory duct, uterus) and storage organs (SR, spermathecae). In contrast, a corkscrew-like helical waveform is ideal for rapid advancing movement and suspected for traveling through long tubules that interconnect these reservoirs. Here, we describe new methods for capturing these complex sperm movements that naturally occur in the reproductive tract. The imaging methods coupled with large mutant collections and genomic resources make the fly sperm a powerful new model for understanding flagellar motility and its dynamic regulation in vivo. The motility regulatory proteins we have identified in the fly are broadly conserved, thus illustrating a general utility of this model system. © 2013 Elsevier Inc. All rights reserved.