Green fluorescent protein as a reporter of transcription and protein localization in fungi

Abstract

Green fluorescent protein (GFP) is a versatile and powerful tool for analysis of diverse biological processes. The recent development of GFP variants with altered spectral properties and altered codon composition has allowed efficient expression of GFP in a number of fungal species. GFP has been successfully used to analyze transcription regulation as well as protein and organelle localization, and promises to give an unprecedented view into the dynamic subcellular processes that shape the fungal cell.