Purification of Immunoglobulin G

Abstract

IgG can be purified, as described here, by ammonium sulfate precipitation followed by size‐exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A‐ and protein G‐affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti‐rat antibody is provided for purification of rat antibodies. Ion‐exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.