Study on the analysis of methamphetamine and its metabolite amphetamine in blood and urine

Abstract

The methods using GC/MS,GC/FID,GC/NPD for identification and quantification of Methamphetamined(MAMP) and Amphetamine (AMP) in human blood and urine were developed. Using 4-phenylbutylamine as the internal standard, the samples were extracted with 200 microliters cyclohexane and then the 2 microliters cyclohexane was injected into GC or the extracts were derivaized by using microwave irradiation. The methods given allowed simple and rapid procedure, the recoveries were greater than 80% and the sensitivity limits were 2-5 ng/ml. The methods for d/L Enantiomer of MAMP and AMP in human urine was also described, which can be used to determine the source of Amphetamines and determine the toxic effect in the case.