Study of spatial distribution of protein bindings in situ by means of reactive dyes.

Abstract

A set of six reactive dyes (RD) was suggested, which are covalently bound with -NH2 and >NH groups in any protein bindings (PB), as well as systems of solvents, in which PB are dyed in volume, and the combination of RD with minerals, lipids, and polysaccharides is excluded. An experimental testing of RD and systems of solvents was carried out on models with different distribution, concentration, and aging degree of PB; on different minerals and their mixtures with lipids and polysaccharides; on samples with different combinations of PB with lipids and polysaccharides. The reliability and selectivity of dyeing were compared for RD and acid dyes. It was shown that RD in the investigated systems of solvents can be universal agents for a selective visualization of spatial distribution of PB (films, filling of large cavities, mixtures with minerals) with any painting technique and degree of aging.