Model sequences for evaluation of the GC dimer sequence repetition on synthesis success were prepared and analyzed by HPLC. Contiguous d(G-C) or d(C-G) sequences have a deleterious effect on DNA oligonucleotide synthesis. The critical number seems to be about 6 GCs in a row. If the GCs are separated by other nucleotides, the effect is not as severe. Copyright © Taylor & Francis Group, LLC.
CITATION STYLE
Hachmann, J., & Lebl, M. (2006). Synthesis of poly d(G-C) oligonucleotides. Nucleosides, Nucleotides and Nucleic Acids, 25(7), 705–717. https://doi.org/10.1080/15257770600725903
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