We used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as α-gustducin, Gγ13, phospholipase C-β2 (PLC-β2) and inositol 1, 4, 5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds.
CITATION STYLE
Pérez, C. A., Huang, L., Rong, M., Kozak, J. A., Preuss, A. K., Zhang, H., … Margolskee, R. F. (2002). A transient receptor potential channel expressed in taste receptor cells. Nature Neuroscience, 5(11), 1169–1176. https://doi.org/10.1038/nn952
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