Uncovering the complexity of Transcriptomes with RNA-SEQ

Abstract

It is commonly known that the genetic information is conveyed from DNA to proteins via the messenger RNA (mRNA) through a finely regulated process. To achieve such a regulation, the concerted action of multiple cis-acting proteins that bind to gene flanking regions-“core” and “auxiliary” regions-is necessary [1]. In particular, core elements, located at the exons’ boundaries, are strictly required for initiating the pre-mRNA processing events, whereas auxiliary elements, variable in number and location, are crucial for their ability to enhance or inhibit the basal splicing activity of a gene.