Computer programs for the analysis of protein fluorescence quenching data

Abstract

Educational computer programs for analysis of fluorescence quenching studies of proteins have been developed. The program is written according to the classical Stern-Volmer equation and some modified Stern-Volmer equations. The static and dynamic quenching constants as well as the accessibility of the quencher molecules to the fluorescence groups are calculated. The experimental data are plotted on a high resolution graph. The calculated data or the graphs can be printed out to obtain a hard copy for filing. © 1987.