Estimation of resistant starch content of high-amylose corn starch

Abstract

Resistant starch (RS) of autoclaved and cooled amylomaize VII starch was simply estimated from direct correlation between RS and a-amylase resistant starch (A-RS) of either mixtures of Novelose 330 and Corn starch (mixture I) or Novelose 330 and amytomaize VII starch (mixture II). Correction factors of 1.17 and 1.14 were required for mixture I and mixture II, respectively, to convert the estimated RS value into AOAC RS content. Swelling power and Rapid Visco Analyser (RVA) peak viscosity of the heat-treated starch and the mixtures linearly decreased as RS content increased. RS content of heat-treated starch estimated by the relationship between RS and A-RS of the mixtures was more accurate than that obtained by the relationship between RS and swelling power or RVA peak viscosity of the mixtures.