Abstract
The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.
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CITATION STYLE
Riabinina, O., Luginbuhl, D., Marr, E., Liu, S., Wu, M. N., Luo, L., & Potter, C. J. (2015). Improved and expanded Q-system reagents for genetic manipulations. Nature Methods, 12(3), 219–222. https://doi.org/10.1038/nmeth.3250
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