A new validated RP-HPLC method for the estimation of capecitabine in bulk and pharmaceutical dosage forms

Abstract

A simple and accurate RP-HPLC method has been developed for the estimation of Capecitabine in bulk and pharmaceutical dosage forms, using C8 Hypersil BDS (Base Deactivated Silane) 250 × 4.6 × 5μ particle size in isocratic mode, with mobile phase comprising of buffer (pH 2.3-2.5) and Acetonitrile in the ratio of 80:40 v/v. The flow rate was 1.2 ml/min and the detection was monitored out by UV detector at 240nm. The retention time for Capecitabine was found to be 5.825min.The proposed method has permitted the quantification of Capecitabine over linearity in the range of 20-120 μg/ml and its percentage recovery was found to be 99.96-100.32 %. The intra day and inter day precision were found 0.16% and 0.10%, respectively.