A program to compute the sizes and numbers of spherical bodies from observations made on tissue sections

Abstract

A computer program, written in standard Fortran, is given to facilitate the computation of the sizes and correct distribution of spheres from the number and size of the circular profiles observed in tissue sections. The method is particularly valuable in cases where it is necessary to determine absolute numbers of cells or subcellular fragments where the objects of interest are not of uniform size. The method gives true cell numbers and the distribution of the spherical diameters. © 1977 Chapman and Hall Ltd.