Structure and function of an rna-reading thermostable dna polymerase

Abstract

Unfaithful: The structure of a DNA-dependent DNA polymerase, whose parental ancestor has no significant reverse transcriptase activity, was obtained for the first time incorporating a nucleotide against an RNA template and then structurally characterized. The DNA polymerase variant has significant reverse transcriptase activity that allows its application in PCR-based molecular diagnostics. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.