TEXT BOOK OFBIOCHEMISTRY
For 2nd year Medical Laboratory Technician Course
(INTERMEDIATE VOCATIONAL)
Author :
S. Srinivasa Rao, B.Pharmacy,
Jr. Lecturer in M.L.T.,Alluri Sri Rama Raju Govt. Junior College,Shanthi Nagar, Khammam.
Editor :
S. Kamalakar Rao, M. Pharmacy
Senior Lecturer in Pharmacy,Department of Biotechnology,Govt. institute for post diploma courses in engineering and technology,Ramanthapur,Hyderabad.

INDEX
Chapter Page No.
I. Instrumental methods of Biochemical analysis ..................... 1
1. Flame Photometry ................................................................... 1
2. Fluorimetry ........................................................................... 10
3. Nephelometry ....................................................................... 12
4. Basic principles and applications of Potentiometry .................. 16
II. Separation techniques ............................................................ 25
1. Chromatography ................................................................... 25
2. Electrophoresis ..................................................................... 53
III. Immunoassays ........................................................................ 70
1. Radio immunoassays ............................................................. 70
2. Enzyme linked immunoassays ................................................ 89
IV. Metabolism ............................................................................. 94
1. Carbohydrate metabolism...................................................... 95
2. Lipid metabolism ................................................................. 101
3. Protein metabolism .............................................................. 104
V. Titrimetric methods of quantitative determination, Preparation
of varioussolutions used in Titrimetric analysis .............. 109
VI. Liver function tests .............................................................. 153
1. Basic concepts including normal and abnormal
bilirubin metabolism .............................................................. 153
2. Classification ............................................................................. 157
3. Serum Bilirubin determination .............................................. 158
4. Vandenbergh test ................................................................. 166
5. Total proteins and A/G ratio.............................................. 167
6. Enzyme estimations as LFT ................................................. 173
VII.Renal function tests ............................................................. 178
1. Basic concepts and Classification........................................ 178
2.Clearance tests ..................................................................... 183
3.Concentration and dilution tests ............................................ 186
4.Urine examination in assessing kidney function....................... 187
VIII.Gastric function tests ......................................................... 217
1. Basic concepts and introduction........................................... 217
2. Techniques of different tests including
Tubeless Gastric analysis ..................................................... 222
IX.Thyroid function tests ........................................................... 233
1. Basic concepts .................................................................... 233
2. Estimations of various thyroid hormones,
their interpretations ............................................................. 235
3. Recent methods of Thyroid function tests ............................. 236
X.Pancreatic function tests ........................................................ 239
1. Basic concepts and introduction........................................ 239
2. Various tests done and methods including
serum amylase determination ................................................... 242
XI.Clinical Enzymology ............................................................. 249
1. Introduction and Basic concepts of
Enzymes Coenzymes and Isoenzymes ................................ 249